Stem cells on a scaffold
Scaffolds are popular in biomedical applications due to their very high surface area and the possibility of growing microorganisms and cells. However, their high surface area is a challenging case for AFM topography study since there is a high chance of destroying the probe or the sample during navigation.
Therefore, the AFM-in-SEM technique enabled us to localise to the region of interest using SEM and further provide a detailed surface topography study using AFM.
- fast ROI identification and safe tip navigation
- SEM material contrast of cell/scaffold
- cell morphology study – topography, membrane roughness, stiffness
Sample courtesy of Lucy Vojtová, CEITEC