Control of nanoparticle cellular uptake and therapeutic efficacy through YAP signaling

The study aims to enhance therapeutic delivery by delving into the interactions between cells and nanoparticles. It finds that inhibiting the Yes-associated protein (YAP) boosts the uptake of nanoparticles in triple-negative breast cancer cells. This suggests that targeting YAP could make these cells more responsive to chemotherapy, thereby increasing the effectiveness and selectivity of nanotherapies.

Furthermore, the research utilizes Correlative Probe and Electron Microscopy (CPEM) via AFM-in-SEM LiteScope to examine the membrane structure of YAP-deficient (YAP -/-) CAL51 cells. It reveals notable differences in morphology and mechanics compared to the wild-type control. This method integrates Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM) for in-situ characterization of 3D surfaces, assessing surface roughness, and conducting height/depth profiling with precise navigation of the AFM tip.

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